quantikine high sensitivity hs human il 17 immunoassay kit  (R&D Systems)

Journal: Frontiers in Pediatrics

Article Title: Cytokine responses to SARS-COV2 infection in mother-infant dyads: a systematic review and meta-analysis

doi: 10.3389/fped.2023.1277697

Figure Lengend Snippet: Characteristics of included studies.

Article Snippet: Tartaglia et al. ( ) , Italy , Prospective case-control study , COVID recovered Pregnant women (pCOV), n = 17 , COVID recovered matched non- pregnant women (nCOV), n = 12 , Maternal serum samples, no timing mentioned , 11 cytokines, chemokines, and growth factors- GM-CSF, IFN-gamma, IL-1 beta/ IL-1F2, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 p70, IL-13, TNF-alpha, IL-17 , Luminex Human Th1/Th2 11-plex Fixed Panel, Human IL-17 immunoassay, biotechne.

Techniques: Enzyme-linked Immunosorbent Assay, Electrochemiluminescence, Luminex, Infection, Flow Cytometry

Journal: PLOS One

Article Title: Clinical proof of concept for small molecule mediated inhibition of IL-17 in psoriasis

doi: 10.1371/journal.pone.0341049

Figure Lengend Snippet: (A) Inhibition of IL-17 bioactivity as secreted by human TH17 cells and assayed by a reporter cell assay. Dotted lines represent positive and negative assay controls. 1 representative of 7 experiments with different donors is shown. The error bars represent the SEM. (B, C) Evaluation of DC-806 in rat CIA. Rats were randomized on Day 11 and dosed as indicated. Daily measurements of ankle thickness (B) and terminal measurement of footpad weights (C) were used as efficacy readouts. (D) Preclinical evaluation of DC-806 serum levels. PK sampling for exposure determination was performed on Days 11, 16, at 1 and 12 hours post dose. The dotted lines represent the uncorrected IC 50 for rat IL-17AA and rat IL-17AF as listed in . The dashed line represents the LLQ of the quantification assay. All error bars represent the SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Abbreviations : anti-F, anti-IL-17F; BID, twice daily; CIA, collagen-induced arthritis; Dex, dexamethasone; IC 50 , half-maximal inhibitory concentration; IL-17, interleukin-17; LLQ, lower limit of quantification; PK, pharmacokinetic; QD, once daily; SEM, standard error of mean; TH17, T-helper 17.

Article Snippet: Serum IL-17A level was measured using Quantikine ® High Sensitivity (HS) Human IL-17 Immunoassay kit #HS170 (R&D Systems), which mostly detects IL-17AA with approximately 3% cross-reactivity to IL-17AF [ ].

Techniques: Inhibition, Sampling, Concentration Assay

Journal: PLOS One

Article Title: Clinical proof of concept for small molecule mediated inhibition of IL-17 in psoriasis

doi: 10.1371/journal.pone.0341049

Figure Lengend Snippet: Graph detailing the group mean ± SD DC-806 plasma concentration by 200 mg BID (green circle) and 800 mg BID (blue diamond) groups on a semilogarithmic scale through Study Day 29. PK in psoriasis patients was comparable to healthy volunteer PK, with consistent trough levels over 28 days after achieving steady state around Day 3. The dotted line represents IC 50 for recombinant human IL-17AA, as obtained using HEK-blue IL-17 cell-based assay . PK Concentration Analysis Set. Abbreviations : BID, twice daily; HEK: human embryonic kidney; IC 50 , half-maximal inhibitory concentration; IL-17, interleukin-17; PK, pharmacokinetic; SD, standard deviation.

Article Snippet: Serum IL-17A level was measured using Quantikine ® High Sensitivity (HS) Human IL-17 Immunoassay kit #HS170 (R&D Systems), which mostly detects IL-17AA with approximately 3% cross-reactivity to IL-17AF [ ].

Techniques: Clinical Proteomics, Concentration Assay, Recombinant, Cell Based Assay, Standard Deviation

Journal: PLOS One

Article Title: Clinical proof of concept for small molecule mediated inhibition of IL-17 in psoriasis

doi: 10.1371/journal.pone.0341049

Figure Lengend Snippet: (A–C) DC-806 demonstrated biological effects as evidenced by dose-dependent responses of biomarkers of interest compared to placebo. Blue diamonds represent the 800 mg BID group; Green circles represent the 200 mg BID group; Black triangles represent the placebo group. (A) Change in mean ± SEM serum IL-17AA levels from time of first dose through 28 days post first dose. The dotted line represents low limit of quantification of the assay. (B) Change in mean ± SEM serum BD-2 levels from time of first dose through 28 days thereafter. *Except on Day 28 when n = 10; **Except on Day 28 when n = 9. (C) Change in mean ± SEM plasma IL-19 levels from time of first dose through 28 days post first dose. The dotted line represents normalization value of 21 pg/mL. PD Biomarker Analysis Set. Abbreviations: BD-2, beta defensin-2; BID, twice daily; BL, baseline; IL-17, interleukin-17; PASI, psoriasis area and severity index; PD, pharmacodynamic; SEM, standard error of mean.

Article Snippet: Serum IL-17A level was measured using Quantikine ® High Sensitivity (HS) Human IL-17 Immunoassay kit #HS170 (R&D Systems), which mostly detects IL-17AA with approximately 3% cross-reactivity to IL-17AF [ ].

Techniques: Clinical Proteomics, Biomarker Discovery

Journal: Archives of biochemistry and biophysics

Article Title: Evaluation of the activity of cardiac glycosides on RORγ and RORγT nuclear receptors.

doi: 10.1016/j.abb.2024.110085

Figure Lengend Snippet: Fig. 7. The impact of selected cardiac glycosides on the expression of genes crucial for the anticancer activity of human Th17 cells. Human naive CD4+ cells were exposed to marinobufagenin (2.5 μM), bufalin (0.005 μM), oleandrin (0.01 μM), telecinobufagin (0.03 μM), and ouabain (0.04 μM) and cultured under Th17- polarizing conditions for 5 days. Subsequently, the cells were harvested for RNA extraction, and the expression of the IL17A, IL17F, GZMA, GZMB, IFNG, CCR6, CXCL10, and CCL20 genes was determined using real-time RT–PCR. The results were normalized to the housekeeping genes HPRT1, HMBS, and RPL13A. An asterisk indicates a statistically significant difference at p < 0.05 compared with control cells. The data are presented as statistical dot plots with the median values (bars) from nine independent donors (n = 9).

Article Snippet: The levels of IL-17 and IFN-γ in the supernatants of cultured naive CD4+ cells subjected to Th17-polarizing conditions and treated with the I. Karwaciak et al. Archives of Biochemistry and Biophysics 759 (2024) 110085 specified cardiac glycosides were assessed using a Quantikine Human IL17 Immunoassay Kit (R&D Systems, Minneapolis, MN, USA) and a Quantikine Human IFN-γ Immunoassay Kit (R&D Systems).

Techniques: Expressing, Activity Assay, Cell Culture, RNA Extraction, Quantitative RT-PCR, Control

Journal: Archives of biochemistry and biophysics

Article Title: Evaluation of the activity of cardiac glycosides on RORγ and RORγT nuclear receptors.

doi: 10.1016/j.abb.2024.110085

Figure Lengend Snippet: Fig. 8. Quantification of IL-17 (A) and IFN-γ (B) production in the supernatants of Th17 cells cultured with (2.5 μM), bufalin (0.005 μM), oleandrin (0.01 μM), telecinobufagin (0.03 μM), and ouabain (0.04 μM) for 4 days was performed using a Quantikine Human IL-17 Immunoassay Kit (R&D Systems) and Quan tikine Human IFN-γ Immunoassay Kit (R&D Systems), respectively. The results are displayed as statistical dot plots with median values indicated by bars and derived from data collected from nine independent donors (n = 9).

Article Snippet: The levels of IL-17 and IFN-γ in the supernatants of cultured naive CD4+ cells subjected to Th17-polarizing conditions and treated with the I. Karwaciak et al. Archives of Biochemistry and Biophysics 759 (2024) 110085 specified cardiac glycosides were assessed using a Quantikine Human IL17 Immunoassay Kit (R&D Systems, Minneapolis, MN, USA) and a Quantikine Human IFN-γ Immunoassay Kit (R&D Systems).

Techniques: Cell Culture, Derivative Assay